????? 超氧化物歧化酶對機(jī)體的氧化與抗氧化平衡起著至關(guān)重要的作用。此酶能清除超氧陰離子自由基，保護(hù)細(xì)胞免受損傷，本試劑盒采用黃嘌呤氧化酶法(羥胺法)測定SOD活力。可測血清(漿)、腦脊液、胸水、腹水、腎透析液、尿液、精液、紅細(xì)胞、白細(xì)胞、血小板、心肌培養(yǎng)細(xì)胞、腫瘤培養(yǎng)細(xì)胞、各種動(dòng)植物組織細(xì)胞、及亞細(xì)胞水平(線粒體、微粒體)中的SOD活力，并可檢測微生物、藥物、食品、飲料、化妝品中的SOD活力。
產(chǎn)品優(yōu)點(diǎn)如下：
1、快速簡便：全程約50分鐘，可測100例左右樣本。
2、取樣量微：本法取手指或耳垂等末梢血20ul～50ul即可測紅細(xì)胞中的SOD，只需2ml靜脈血可測白細(xì)胞中的SOD及血小板中SOD，只需50mg左右組織就可測組織勻漿、胞漿中的SOD，0.2g組織可測線粒體及微粒體中的SOD。
3、靈敏度高：IC50=0.05g/ml，是鄰苯三酚法的18倍。
4、穩(wěn)定性好：試劑盒2～8℃存放6個(gè)月有效。
5、再現(xiàn)性好：變異系數(shù)CV=1.7%。
6、回收試驗(yàn)： X =103.3%。
7、受外界影響因素小：干擾因素少，重復(fù)性強(qiáng)。
8、測試面廣：可測動(dòng)物血液、組織、各種體液、灌流液等、各種培養(yǎng)細(xì)胞、細(xì)菌、植物組織、各種水產(chǎn)以及化妝品、保健品等，效果均佳。
|
批間CV
|
批內(nèi)CV
|
回收率
|
最底檢出線
|
檢測范圍
|
|
3.52
|
1.7
|
103
|
5.0U/ml
|
5.0-122.1 U/ml
|
本試劑盒保存期：6個(gè)月。貯存溫度：2～8℃。
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